68. Safety Test 2
- INDEX -
Residual Chemicals
Ethylene Dibromide (EDB)
Organo-phosphorus Pesticides
Organochlorine Pesticides
Ethylene Dibromide (EDB)
Ethylene dibromide is a fumigant for cereals, fruits, vegetables, lumber, and soil. It is toxic to humans causing respiratory problems, pulmonary edema, and perception and athletic disorders. It is also known to cause a cancer of the nasal cavity, lung, and breast. As a result, the use of ethylene dibromide has been prohibited in Japan and the United States of America since 1985. In the classification of deleterious substances and poisons, it is grouped as a deleterious substance.
A Dean Stark distillatory apparatus is used to capture EDB, as well as for extraction and clean-up.
1) Refluxing and boiling of sample: Put 50g of unbroken sample grains into a flask, add 200ml of water and 20ml of n-hexane, and reflux and boil it for one hour.
2) Separation: Take water and n-hexane from the trap, add 15g of salt and shake it. Separate n-hexane.
3) Dehydration: Dehydrate n-hexane layer with sodium sulfate anhydride.
4) Test solution: Pass the solution through a florisil cartridge (florisil sep-pak of Millipore Corp.) to make the test solution.
5) Gas chromatographic measurement and testing criteria:
Measuring equipment: ECD gas chromatograph
Column: Fused silica capillary column, DB-20M, 0.53mm inner diameter x 30m long,
film thickness of 1.0μm
Column temperature: 80℃
Sample injection port temperature: 250℃
Detector temperature: 280℃
Injection method: Splitless
Carrier gas: Nitrogen, 0.5kgf/cm2
Make-up gas: Nitrogen, 1.0kgf/cm2
Injection volume: 2 μl
Organo-phosphorus Pesticides
Organo-phosphorus pesticides include the following substances. They are the most commonly used pesticides throughout the world. They are toxic to humans causing disorders of the nerve center and eye tissue, as well as pulmonary edema and breathing abnormalities. Parathion, in particular, is highly poisonous, having caused many cases of intoxication and death, and has been banned in Japan since 1967. These are all classified as deleterious substances, except for parathion, which is classified as a specified poison.
Dichlorvos, diazinon, salithion, chlorpyrifos methyl, pirimiphos-methyl, iprofenphos, chlorpyrifos, fenthion, dimethiofos, prothiofos, malathion, phenthoate, chlorfenvinphos-E, fenitrothion, chlorfenvinphos-Z, parathion, methidathion, ethion, cyanofenphos, EPN, phosalone, etc.